Vacuum Liquid Chromatography of Cystoseira usneoides for purification of antioxidant compounds.

Abstract

Event Abstract Back to Event Vacuum Liquid Chromatography of Cystoseira usneoides for purification of antioxidant compounds. Sylvie Santos1, Joana Silva2, Susete Pinteus2, André Horta2, Celso Alves2 and Rui Pedrosa2* 1 Berufskolleg für Biotechnik in Hohenheim, Landwirtschaftliche Schule Stuttgart Hohenheim, Germany 2 School of Tourism and Maritime Technology - IPL, GIRM, Portugal Cystoseira usneoides is a brown algae commonly found in the Portuguese coast. A previous study has reported high antioxidant activity of its extract, however, few works have been made in order to isolate and identify the molecules responsible for the antioxidant activity. In this work, a vacuum liquid chromatography (VLC) was performed to Cystoseira usneoides dichloromethane extract using silica gel with the following solvents system: 100% cyclohexane followed by a mixture of 3:1; 1:1; 1:3 of cyclohexane and ethyl acetate and 100 % ethyl acetate. The five fractions were concentrated in a rotary evaporator and dissolved in DMSO at 100mg/ml. The antioxidant potential of all fractions was evaluated by both, the quantification of the total phenolic content (TPC) and by the evaluation of the DPPH radical scavenging activity. Comparatively to the initial values of DPPH radical scavenging activity (IC50= 81.8 µg/ml (54.74-122.2)) and the total phenolic content (86.28 ± 0.002 mg of gallic acid eq./g of extract) of the crude dichloromethane extract, all the fractions obtained by VLC in a silica gel column reported much less antioxidant activity. The most active fractions scavenging the DPPH radical were F3, F4 and F5 which presented and IC50 of 1314 (1209-1429), 2084 (1624-2674) and 1414 (1199-1668) µg/ml, respectively. F5 fraction presented the highest phenolic content of 20.85 mg of gallic acid equivalents/g of extract. The results suggest that the compounds we are trying to isolate have strong affinity with the stationary phase, and therefore, weren’t eluted with the solvents, even with the increasing polarity along the chromatography. Even though VLC, with a silica gel column, have already revealed to be efficient for separation of compounds extracted with dichloromethane, as regards to antioxidant compounds, these continue to be a great challenge. Keywords: VLC, Brown algae, Total phenolic content, DPPH radical scavenging activity, Bioactive marine natural compounds Conference: IMMR | International Meeting on Marine Research 2014, Peniche, Portugal, 10 Jul - 11 Jul, 2014. Presentation Type: Poster Presentation Topic: BLUE BIOTECH Citation: Santos S, Silva J, Pinteus S, Horta A, Alves C and Pedrosa R (2014). Vacuum Liquid Chromatography of Cystoseira usneoides for purification of antioxidant compounds.. Front. Mar. Sci. Conference Abstract: IMMR | International Meeting on Marine Research 2014. doi: 10.3389/conf.fmars.2014.02.00051 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 12 May 2014; Published Online: 18 Jul 2014. * Correspondence: Prof. Rui Pedrosa, School of Tourism and Maritime Technology - IPL, GIRM, Peniche, 2520–641, Portugal, rpedrosa@ipleiria.pt Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Sylvie Santos Joana Silva Susete Pinteus André Horta Celso Alves Rui Pedrosa Google Sylvie Santos Joana Silva Susete Pinteus André Horta Celso Alves Rui Pedrosa Google Scholar Sylvie Santos Joana Silva Susete Pinteus André Horta Celso Alves Rui Pedrosa PubMed Sylvie Santos Joana Silva Susete Pinteus André Horta Celso Alves Rui Pedrosa Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.