Cytotoxicity and antiproliferative activity of fractions isolated from Fucus spiralis seaweed

Abstract

Event Abstract Back to Event Cytotoxicity and antiproliferative activity of fractions isolated from Fucus spiralis seaweed Celso Alves1*, Susete Pinteus1, André Horta1, Joana Silva1, Nádia Fino1, Olivier Thomas2 and Rui Pedrosa1 1 School of Tourism and Maritime Technology, Portugal 2 ICN - University of Nice-Sophia Antipolis, France In the last decades, nature has played a significant role as source of new drugs and recent trends in medicines research emphasize that marine environment has a high potential for discovery of new pharmaceutical compounds. Harsh chemical and physical conditions in the marine environment provide a production of quite specific and potent active molecules. The aim of this study was to evaluate the antitumor activity of isolated fractions obtained from seaweed Fucus spiralis on three human tumor cell lines (HepG-2; MCF-7; SH-SY5Y). In this study, five fractions obtained by Vacuum Liquid Chromatography (VLC) from Fucus spiralis methanolic extracts were tested on HepG-2, MCF-7 and SH-SY5Y cells through cytotoxicity and anti-proliferative assays. The results were obtained the according MTT method. The highest cytotoxicity was exhibited by F3 fraction on HepG-2 cells and by F1 and F5 fractions on SH-SY5Y cells, reduced the cells viability (1 mg/ml; 24 hours) in 83%, 97% and 91%, respectively. The smallest IC50 was exhibited by F1 fraction on SH-SY5Y with 308.0µg/ml. In cell proliferation assays, F1, F3, F4 and F5 fractions showed anti-proliferative activity on all cell lines tested. (1 mg/ml; 24 hours). However, the effects were more marked on HepG-2 and SH-SY5Y cells. The smallest IC50 was exhibited on SH-SY5Y cells by F1 and F3 fraction with an IC50 of 153.2 and 136.4µg/ml, respectively. In conclusion, Fucus spiralis is a promising source of new molecules with therapeutically applications in cancer. Acknowledgements This work was supported through funding provided by the European Commission Collaborative Project FP7 KBBE 2010–4 BAMMBO (Project Number 265896). Keywords: algae, antitumor activity, bioactive compounds, Marine Natural Products, MCF-7 Cells, HepG2 cells, SH-SY5Y cells Conference: IMMR | International Meeting on Marine Research 2014, Peniche, Portugal, 10 Jul - 11 Jul, 2014. Presentation Type: Poster Presentation Topic: BLUE BIOTECH Citation: Alves C, Pinteus S, Horta A, Silva J, Fino N, Thomas O and Pedrosa R (2014). Cytotoxicity and antiproliferative activity of fractions isolated from Fucus spiralis seaweed. Front. Mar. Sci. Conference Abstract: IMMR | International Meeting on Marine Research 2014. doi: 10.3389/conf.fmars.2014.02.00067 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 10 May 2014; Published Online: 18 Jul 2014. * Correspondence: Dr. Celso Alves, School of Tourism and Maritime Technology, Peniche, Portugal, celso.alves@ipleiria.pt Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Celso Alves Susete Pinteus André Horta Joana Silva Nádia Fino Olivier Thomas Rui Pedrosa Google Celso Alves Susete Pinteus André Horta Joana Silva Nádia Fino Olivier Thomas Rui Pedrosa Google Scholar Celso Alves Susete Pinteus André Horta Joana Silva Nádia Fino Olivier Thomas Rui Pedrosa PubMed Celso Alves Susete Pinteus André Horta Joana Silva Nádia Fino Olivier Thomas Rui Pedrosa Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.