Abstract
A method has been developed to measure DNA synthesis in protoplasts freshly isolated from bromegrass cell suspension culture. The method involves exposing the protoplasts to a transient hypotonic shock in the presence of magnesium, substrate deoxyribonucleotides and ATP as an energy source. Incorporation was linear for up to 45 min and was not reduced by addition of thymidine. The optimal pH was 6.5. Incorporation was decreased by over half in the presence of aphidicolin, a potent inhibitor of replication.
Full Text
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call