Use of Nuclear Imaging in tracking of adoptive Treg therapy in transplantation: a pre-clinical model.

Abstract

Event Abstract Back to Event Use of Nuclear Imaging in tracking of adoptive Treg therapy in transplantation: a pre-clinical model. Ehsan Sharif-Paghaleh1, 2*, John Leech1, Robert Lechler1, Lesley Smyth1, Gregory Mullen1, 2 and Giovanna Lombardi1 1 King's College London, MRC Center for Transplantation, United Kingdom 2 King's College London, Imaging Sciences, United Kingdom Regulatory T cells (Tregs) are key in controlling autoimmune diseases and limiting immune responses to foreign antigens. Tregs are being studied for adoptive transfer immunotherapy for various diseases such preventing transplant rejection. However, key questions such as where therapeutic Tregs go and how long they stay viable in patients remains unsolved. Here we are trying to answer these questions in a pre-clinical set up with the help of nuclear medicine imaging technology. Imaging of the human sodium/iodide symporter (NIS) via Single Photon Emission Computed Tomography (SPECT) has been used to image various cell types in vivo. This study addresses whether SPECT/CT imaging can be used to visualise the migratory pattern of Tregs in vivo. Murine Tregs were transduced with a construct encoding for NIS. NIS expressing Tregs were specifically radiolabelled in vitro with Technetium-99m pertechnetate (99mTcO4-) and exposure of these cells to radioactivity did not affect cell viability, phenotype or function. Moreover, we have demonstrated that this method of imaging can be utilised to image migration of Tregs with direct and indirect allo-specificity in a skin transplant model. Interestingly, Tregs that prolonged the transplant survival demonstrated a different pattern of migration compared to Tregs that were unable to prolong the transplant survival. The data presented here suggests that SPECT/CT can be utilised in preclinical imaging studies of adoptively transferred Tregs without affecting Treg function and viability thereby allowing longitudinal studies within disease models. Moreover, this technology has also the potential to be applied to human Treg studies in future. Keywords: Treg, adoptive therapy, imaging, spect-ct, Transplantation, Whole Body Imaging Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Translational immunology and immune intervention Citation: Sharif-Paghaleh E, Leech J, Lechler R, Smyth L, Mullen G and Lombardi G (2013). Use of Nuclear Imaging in tracking of adoptive Treg therapy in transplantation: a pre-clinical model.. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00780 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 17 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Ehsan Sharif-Paghaleh, King's College London, MRC Center for Transplantation, London, United Kingdom, ehsan.sharif-paghaleh@kcl.ac.uk Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Ehsan Sharif-Paghaleh John Leech Robert Lechler Lesley Smyth Gregory Mullen Giovanna Lombardi Google Ehsan Sharif-Paghaleh John Leech Robert Lechler Lesley Smyth Gregory Mullen Giovanna Lombardi Google Scholar Ehsan Sharif-Paghaleh John Leech Robert Lechler Lesley Smyth Gregory Mullen Giovanna Lombardi PubMed Ehsan Sharif-Paghaleh John Leech Robert Lechler Lesley Smyth Gregory Mullen Giovanna Lombardi Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.