Abstract
Factors affecting the chromatographic behaviour of the biogenic amines, adrenaline, noradrenaline, dopamine and 5-hydroxytryptamine, and the products of their caeruloplasmin-catalysed oxidation are discussed. These findings are then used to develop an isocratic separation of these eight compounds within a limited time period. This permits the study of the oxidation of biogenic amine mixtures by caeruloplasmin, which cannot be done by conventional enzyme assay methods such as spectrophotometry or polarography. Finally, the results obtained from the oxidation of a mixture of all four amines by caeruloplasmin are presented and discussed.
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