Abstract
Aspergillus niger is widely used as a homologous and heterologous protein expression host in the food industry. Therefore, there is an emergent need to construct a highly efficient expression vector. The promoter region encoding the β-glucosidase II (GQ471881) was evaluated. Specifically, two CCAAT-box, one CREA, and one AREA sequence located at −343 to −339nt, −159 to −155nt, −226 to −221nt, and −63 to −60nt (relative to the start codon of the bglII gene), respectively, were studied. The function of these consensus sequences was investigated by deletion analysis of the bglII promoter fused with the uidA gene (β-glucuronidase, GUS) as reporter. The GUS specific activity of the transformant, containing the hole region (−402 to −1) of the promoter sequence pBARGEM7-2-GUS-ANUT(d0), was 189U/mg. Moreover, the transformant pBARGEM7-2-GUS-ANUT3 (d3), containing −189 to −1 bglII gene promoter region, produced the highest GUS specific activity, 448U/mg. Because transformant d3 did not contain the CREA, it was free from carbon catabolite repression. This promoter has the potential to be an expression vector for application to the food industry.
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