Abstract
Vesicles are thought to play an important role in the polarized apical extension of fungal hyphae. These vesicles are likely to contain cell wall precursors and other molecules destined for exocytosis at the hyphal apex, including polysaccharides and/or other glycosylated molecules which should be recognized by certain lectins, including Concanavalin A (ConA). Here we describe the presence of ConA binding sites (CABS) within the apical vesicles of Trichoderma viride. In addition, we compare single- and double-sided ConA labeling patterns to assess the impact of these different techniques on the interpretation of results.Somatic hyphae of T. viride were grown as a cell monolayer over pieces of cellophane on the surface of nutrient agar plates. The cellophane pieces and cells were plungefrozen in a liquid propane/ethane mixture, freeze-substituted in 2% OsO4 in acetone,and subsequently embedded in Quetol resin. Thin sections were picked up with empty, formvar-dipped, single-slot, gold grids.
Published Version
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