Up-Regulation of miR-130b-3p Activates the PTEN/PI3K/AKT/NF-κB Pathway to Defense against Mycoplasma gallisepticum (HS Strain) Infection of Chicken.

Bo Yuan,Kang Zhang,Yabo Zhao,Yingfei Sun,Mengyun Zou,Xiuli Peng

doi:10.3390/ijms19082172

Abstract

Mycoplasma gallisepticum (MG) is the pathogen of chronic respiratory disease (CRD), hallmarked by vigorous inflammation in chickens, causing the poultry industry enormous losses. miRNAs have emerged as important regulators of animal diseases. Previous miRNA sequencing data has demonstrated that miR-130b-3p is up-regulated in MG-infected chicken embryo lungs. Therefore, we aimed to investigate the function of miR-130b-3p in MG infection of chickens. RT-qPCR results confirmed that miR-130b-3p was up-regulated both in MG-infected chicken embryo lungs and chicken embryonic fibroblast cells (DF-1 cells). Furthermore, functional studies showed that overexpression of miR-130b-3p promoted MG-infected DF-1 cell proliferation and cell cycle, whereas inhibition of miR-130b-3p weakened these cellular processes. Luciferase reporter assay combined with gene expression data supported that phosphatase and tensin homolog deleted on chromosome ten (PTEN) was a direct target of miR-130b-3p. Additionally, overexpression of miR-130b-3p resulted in up-regulations of phosphatidylinositol-3 kinase (PI3K), serine/threonine kinase (AKT), and nuclear factor-κB (NF-κB), whereas inhibition of miR-130b-3p led to the opposite results. Altogether, upon MG infection, up-regulation of miR-130b-3p activates the PI3K/AKT/NF-κB pathway, facilitates cell proliferation and cell cycle via down-regulating PTEN. This study helps to understand the mechanism of host response to MG infection.

Highlights

Mycoplasma is the simplest and smallest prokaryote widespread in nature, and is distinguished by the absence of a cell wall [1]
We found that miR-130b-3p could regulate cell proliferation and cell cycle in host defense against Mycoplasma gallisepticum (MG) infection by regulating the phosphatidylinositol-3 kinase (PI3K)/AKT/nuclear factor-κB (NF-κB) pathway through directly targeting PTEN
A previous deep sequencing revealed that miR-130b-3p was overexpressed in MG-infected chicken embryo lungs

Summary

Introduction

Mycoplasma is the simplest and smallest prokaryote widespread in nature, and is distinguished by the absence of a cell wall [1]. It can invade and survive in a wide range of eukaryotes, such as humans, plants, and domestic animals, leading to multiple diseases [2,3,4]. Studies have shown that MG is capable of resisting antibiotics, evading host immune system, and crossing through the respiratory mucosal barrier to establish a systemic infection [6]. The MG-HS strain, used in this study, is a pathogenic strain obtained from a chicken farm in Hubei Province of China [13,14]

Objectives

Methods

Results