Gga-miR-146c Activates TLR6/MyD88/NF-κB Pathway through Targeting MMP16 to Prevent Mycoplasma Gallisepticum (HS Strain) Infection in Chickens

Kang Zhang,Zaiwei Wang,Xiuli Peng,Yun Han,Yali Fu,Yabo Zhao

doi:10.3390/cells8050501

Kang Zhang, Zaiwei Wang + Show 4 more

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https://doi.org/10.3390/cells8050501

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Journal: Cells	Publication Date: May 24, 2019
Citations: 14	License type: CC BY 4.0

Affiliation: Huazhong Agricultural University

Abstract

Mycoplasma gallisepticum (MG), a pathogen that infects chickens and some other birds, triggers chronic respiratory disease (CRD) in chickens, which is characterized by inflammation. The investigation of microbial pathogenesis would contribute to the deep understanding of infection control. Since microribonucleic acids (miRNAs) play a key role in this process, gga-mir-146c, an upregulated miRNA upon MG infection, was selected according to our previous RNA-sequencing data. In this paper, we predicted and validated that MMP16 is one of gga-miR-146c target genes. Results show that MMP16 is the target of gga-miR-146c and gga-miR-146c can downregulate MMP16 expression within limits. gga-miR-146c upregulation significantly increased the expression of TLR6, NF-κB p65, MyD88, and TNF-α, whereas the gga-miR-146c inhibitor led to an opposite result. gga-miR-146c upregulation effectively decreased apoptosis and stimulated DF-1 cells proliferation upon MG infection. On the contrary, gga-miR-146c inhibitor promoted apoptosis and repressed the proliferation. Collectively, our results suggest that gga-miR-146c upregulation upon MG infection represses MMP16 expression, activating TLR6/MyD88/NF-κB pathway, promoting cell proliferation by inhibiting cell apoptosis, and, finally, enhancing cell cycle progression to defend against host MG infection.

Highlights

Mycoplasma, a prokaryotic parasite, has negative impacts on human health and livestock farming [1,2,3].Of those, Mycoplasma gallisepticum (MG) could cause severe chronic respiratory disease (CRD) in chickens and sinusitis in turkeys, represented by severely inflamed tracheas, lungs, and air sacs [4,5]
3.1. gga-miR-146c Expression Was Upregulated after MG Infection ed gga-miR-146c expression in chicken embryos upon MG-HS infection on the ninth
To explore biological functions of gga-miR-146c in MG-HS infection, we focused on influences of gga-miR-146c on cell proliferation, apoptosis, and cycle

Summary

Introduction

Mycoplasma, a prokaryotic parasite, has negative impacts on human health and livestock farming [1,2,3]. Mycoplasma gallisepticum (MG) could cause severe chronic respiratory disease (CRD) in chickens and sinusitis in turkeys, represented by severely inflamed tracheas, lungs, and air sacs [4,5]. Controlling the impact of the disease on a global level is done by vaccination and medication. They cannot eliminate all pathogens of infected chickens, and some vaccines may revert to virulence [6,7,8]. MG infection results in considerable losses consisting of reducied weight gain and egg production, as well as increased embryo morbidity [9].

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