Abstract
An encapsulated formulation of Bacillus thuringiensis (Bt) was produced by the Pickering emulsion technique to improve its activity and stability under UV-A radiation. In this technique latex particles, GO nanosheets, olive oil, ethanol, and water were used to encapsulate Bt in colloidosomes. The protective efficacy of this formulation in protecting Bt subsp. Kurstaki against deactivation by UV-A irradiation was measured, so that spore viability and mortality on Ephestia kuehniella (E. kuehniella) Zeller larvae under UV-A radiation are investigated. According to the results of both tests, encapsulated formulation at a concentration of 0.045% has the highest protection of viability. Hence, colloidosome microcapsule formulations successfully provide good protection against UV radiation.
Highlights
An encapsulated formulation of Bacillus thuringiensis (Bt) was produced by the Pickering emulsion technique to improve its activity and stability under UV-A radiation
We examined the effect of colloidosomes formed using Pickering emulsions, in which Bt was successfully encapsulated in colloidosomes using latex particles, graphene oxide (GO) nanosheets, olive oil, ethanol, and water
The main objective was the development of biological agents to improve the protective effect of the GO nanosheets on the stability of formulations exposed to UV radiation
Summary
An encapsulated formulation of Bacillus thuringiensis (Bt) was produced by the Pickering emulsion technique to improve its activity and stability under UV-A radiation. In this technique latex particles, GO nanosheets, olive oil, ethanol, and water were used to encapsulate Bt in colloidosomes. We examined the effect of colloidosomes formed using Pickering emulsions, in which Bt was successfully encapsulated in colloidosomes using latex particles, GO nanosheets, olive oil, ethanol, and water. Among various materials and techniques that have been developed to fabricate colloidosomes, we report pH-responsive colloidosomes containing Bt crystals that were formed by Pickering emulsions and stabilized by GO nanosheets. The spore viability and the crystal activity of encapsulated formulations were measured
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