Abstract
Over the past decade, synthetic nanopores in solid-state membranes have gained reputation as platforms for studying the biophysical properties of biopolymers. In this presentation, the precise fabrication of ultrathin nanopores and their utility for analyzing complex nucleic acid samples will be discussed, with major emphasis on their resolution capabilities for different biopolymers. The membrane thickness was found to play an important role on the signal obtained from different biopolymers. These findings are critical for developing ultrasensitive nanopore assays which profile biopolymer structure, important for genomics and other biophysical studies.
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