Abstract
Malignant tumor cells have an enhanced rate of cell division and of metabolic activities of cells. We studied IgG positive immunoblastic sarcoma cells by means of freeze-fracturing and found significant structural differences between these tumor cells and control B-cells.Lymphnodes infiltrated with sarcoma cells were dissected from the cervical areas of two patients. The sarcoma cell suspension was obtained from these lymphnodes. Sarcoma cells had fine granules stained with PAS and mothyl green pyronin in cytoplasma. Most of sarcoma cells were positive fluorescent (SIgG bearing). Alpha-naphthyl-acetate-esterase test was negative or false positive. Single cell suspension of lymphnodes was obtained from patients of non-lymphatic diseases. EAC rosette forming cells and adherent cells to nylon column fiber were used as control B-cells. The pellet of sarcoma cells and control B-cells were fixed with 2. 5% glutaraldehyde solution. Freeze-fracture was done as described previously. Under the electron microscope, EAC rosette forming cells were selected as control B-cells.
Published Version
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