Abstract

The pineal gland, an important component of the neuroendocrine axis [review in Reiter] is known to play an important role in chronobiology and transduction of certain external stimuli. The purpose of the present investigation is to examine the pineal gland of young and aged rats and to determine structural and elemental alterations related to age.28 months-old male Wistar rats and young adult males (2-3 months old) were used. Pineal glands were quickly removed from the brain after decapitation and fixed with 3% gl utaraldehyde in 0.1 M Na cacodylate buffer (1h) and postfixed in 1% OsO4 buffered with 0.1 M Na cacodylate. For ultrastructural localization of calcium the potassium pyroantimonate method was used following the method of Eisenmann et al. Calcium was analyzed using a CAMEBAX mi croanalyser with the TEM mode and fitted with WDS spectrometers.

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