Abstract

The pineal gland of the rat is considered to play an important neuroendocrine role in chronobiology, circadian rhythmicity and transduction of certain external stimuli [1]. Several mammalian species, including man, contain calcified concretions (corpora arenacea, brain sand) in their pineal gland [2,3,4]. The aim of the present study was to examine these concretions in aging rats by means of SEM, TEM, X-ray microanalysis and electron diffraction. Deep-freezing followed by freeze-drying, is a means of tissue preparation that has been shown to be a satisfactory alternative for electron microscopic tissue preparation [5]. An attempt was made to study the mechanism of formation of these structures and to understand their possible role in pineal physiology.

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