Ultrastructural localizations of J chains in the chicken bursa of Fabricius at different stages of development.

Abstract

Before and after hatching, J-chain positive cells (JPC) were observed by immunoelectron microscopy in the chicken bursa of Fabricius. JPC were mostly lymphocytes, but epithelial cells were also detected as JPC. During the embryonic stage, J chains were mostly associated as patches with surface membranes. Furthermore, there was a diffuse localization in the cytoplasm. After hatching, J chains showed a similar subcellular localization as was seen before hatching. However, J chains were frequently detected in the cytoplasm, and rarely on the surface membranes after hatching. Staining intensities by corresponding antisera were stronger in the hatched chickens than in embryos. From these findings one may conclude that J chains are synthesized even at an early stage of B cell differentiation during embryonic life and are continuously produced at the later differentiation stages of B-cell lineage. The increased amounts of J chains estimated by staining intensity seem to coincide with B cell maturation and may correlate with signalling of IgM synthesis.