Abstract
The indirect immunoenzymatic technique was employed to study the ultrastructural localization of influenza virus antigens in infected chick embryo cells. After virus adsorption onto cells, a positive peroxidase reaction is observed on the surface of the cellular membrane. Four hours after infection, ribosomes associated with rough membranes of the endoplasmic reticulum showed an extensive peroxidase reaction. During the period of virions maturation (6–8 hours after infection) a positive peroxidase reaction on the cell membrane and the surface of budding viral particles could be detected. Simultaneously, a positive peroxidase reaction occurred in the region of smooth membranes of the endoplasmic reticulum and on smooth membranes of small vesicles which migrated to the cell membrane.
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