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Abstract
ABSTRACT The highest yield (34%) and imino acid (18.7%) of collagen were obtained from yellowfin tuna (Thunnus albacares) skin using 20 min ultrasound. The collagen was identified as type I collagen according to the results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and its structure remained intact, proved by ultraviolet spectroscopy (UV) and fourier transform infrared spectroscopy (FTIR). Collagen solubility was superior in acidic and low salt environments. Ultrasound cavitation for 20 min improved collagens’ emulsifying (EAI: 78.8 m2/g and ESI: 110.3 min) and foam properties (FA: 151.8% and FS: 133.3%) and water holding capacity (51.9%) by enhancing internal hydrophobic interactions and surface hydrophobicity. 20 min ultrasound resulted in desirable DPPH (61.1%) and ABTS (53.1%) radical scavenging and ferric-reducing antioxidant power (0.078 ABS).
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