Ultrasound-enhanced matrix solid-phase dispersion micro-extraction applying Mesoporous Molecular Sieve SBA-15 for the determination of multiple compounds in Fructus Psoraleae

Abstract

An efficient ultrasound enhanced matrix solid-phase dispersion micro-extraction method was established for the extraction and determination of multiple ingredients (psoralenoside, isopsoralenoside, psoralen, isopsoralen, bavachin, psoralidin, isobavachalcone and bakuchiol) from Fructus Psoraleae applying ultra-high-performance liquid chromatography. The experimental factors affecting the extraction efficiency, including extraction solvent concentration, extraction solvent volume and ultrasound time, were optimized by the design of experiments methodology. Central composite design was applied in this optimization approach as a quadratic model. Under optimum conditions, good linear regression (r2 > 0.9999) and recovery (96.8%–104%, RSD<3.73%) for targets were attained. In addition, the intra-day of analytes was below 4.42% and inter-day precision of analytes was below 2.08%. This proposed method only required small amounts of reagent (0.5 mL) and materials usage (20 mg). Thus, the present study applied the efficient method to exhibit fast preparation and determination of eight ingredients of continents in Fructus Psoraleae.