UJI FITOKIMIA DAN DAYA HAMBAT EKSTRAK DAUN JUWET (Syzygium cumini) TERHADAP PERTUMBUHAN Escherichia coli DAN Staphylococcus aureus ATCC

I Ketut Muksin,Ida Bagus Gede Darmayasa,Kadek Sudarmi

doi:10.24843/jsimbiosis.2017.v05.i02.p03

I Ketut Muksin, Ida Bagus Gede Darmayasa + Show 1 more

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https://doi.org/10.24843/jsimbiosis.2017.v05.i02.p03

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Abstract

Bacterial Escherichia coli dan Staphylococcus aureus ATCCis a normal flora bacteria that naturally exist human body. This bacteria may by pathogenic if it exceeds acertain limits. To prevent the disease caused, need to be overcome by using herbal ingredients one of them is juwet leaves (Syzygiumcumini).The purpose of the study was to determine wheter the extract of juwet leaf (S. cumini) can inhibited the growth of E. coli and S. aureus ATCC and to know compounds contained in juwet leaf extract (S. cumini). This research used diffusion wells methodwith 6 treatments and 4 replications. Date analysis using completely randomized design (CRD) with using ANOVA variance analysis. If the date obtained has a real difference test level at the 5% (P? 0,5) that continue with Duncan test. Concentration of the extract tested was 0%, 5%, 10%, 15%, 25% and 50%. Juwet leaf extract is able to inhibited the growth of E. coli and S. aureus ATCC which is shown by the formation of clear zone. Concentration extract leaf which is effective to inhibit E. coli and S. aureus ATCC is the concentration 50% (18,9 mm) for E. coli and (16,5 mm) for S. aureus ATCC. Screening of phytochemical test of juwet leaf extract positive containing alkaloids, phenolic, steroids and saponins.  Keywords : juwet leaf extract (Syzygium cumini), Escherichia coli, Staphylococcus aureus ATCC

Highlights

Bacterial Escherichia coli dan Staphylococcus aureus ATCCis a normal flora bacteria that naturally exist human body
To prevent the disease caused, need to be overcome by using herbal ingredients one of them is juwet leaves (Syzygiumcumini).The purpose of the study was to determine wheter the extract of juwet leaf (S. cumini) can inhibited the growth of E. coli and S. aureus ATCC and to know compounds contained in juwet leaf extract (S. cumini)
Juwet leaf extract is able to inhibited the growth of E. coli and S. aureus ATCC which is shown by the formation of clear zone

Summary

MATERI DAN METODE Penelitian ini dilakukan di Laboratorium Mikrobiologi

Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Udayana pada bulan Januari 2017. Uji fitokimia ekstrak daun juwet (Syzygium cumini) yaitu pertama uji alkaloid yang dilakukan dengan melarutkan ekstrak ke dalam 10 mL kloroform amoniak, ditambah 0,5 mL H2SO4 kemudian dihomogenkan dan didiamkan sampai terbentuk dua lapisan, diambil bagian lapisan atas kemudian ditetesi pereaksi Meyer sebanyak 1 tetes. Apabila terjadi perubahan warna menjadi kemerahan maka ini menunjukkan hasil positif adanya kandungan senyawa flavonoid pada ekstrak tersebut, sedangkan hasil positif fenolik ditandai dengan terbentuknya cincin hijau sampai keunguan (Santoso et al, 2012). Terakhir uji saponin yaitu ekstrak daun juwet diambil sebanyak 0,1 gram, kemudian dilarutkan dalam 5 mL aquades panas, selanjutnya dikocok kurang lebih 10 detik. Rata-rata diameter zona hambat ekstrak daun juwet (Syzygium cumini) terhadap Escherichia coli dan Staphylococcus aureus ATCC. Untuk hasil pengujian golongan senyawa flavonoid dan terpenoid menunjukkan hasil negatif, (Tabel 2 )dan (Gambar 2)

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