Abstract
plastics on a large scale has an effect on the increase to the amount of world waste, such as High Density Polyethylene (HDPE)and Low Density Polyethylene (LDPE) plastics. This study, tried to isolate Pseudomonas and Ochrobactrum bacteria from varioussoil in dumped soil area and mangrove forest in Bali to find out the degradation ability of the bacteria against HDPE and LDPEplastics. The methods used in this study were pour plate method, Biochemical and morphology identification by using Bergey’sManual of Determinative Bacteriology 9th Edition book as a reference, screening test and the determination of bacterial species byusing Vitek 2 Compact System. Evaluation of degradation result was done by measuring the weight loss of plastics residues. Thisstudy has isolated 27 bacteria that estimated from genus Pseudomonas and Ochrobactrum with characteristics as basil shape, Gramnegative, positive catalase and classified as non-fermenter bacteria. Isolate 2 bacteria which isolated from Suwung dumped soil hasidentified as Ochrobactrum anthropi with the ability of HDPE degradation by 20% and Isolate 24 which isolated from Karangasemdumped soil has identified as Pseudomonas aeruginosa with the ability of HDPE degradation by 18,75%.Keywords : Plastics waste, Biodegradation, High Density Polyethylene and Low Density Polyetene
Highlights
Bahan plastik semakin banyak digunakan dalam dunia industri baik industri makanan, pakaian, transportasi, konstruksi, medis maupun rekreasi selama kurun waktu 3 dekade terakhir ini (Fadlilah dan Maya, 2014)
Plastics classified as multifunctional complex polymer groups
that has become a conventional products in every life sectors
Summary
Penelitian ini tergolong jenis penelitian eksplorasi dan eksperimental dengan proses pengambilan sampel dilakukan pada 2 lokasi berbeda, yaitu di daerah Tempat Pembuangan Akhir (TPA) dan hutan mangrove. Sampel tanah dari 2 lokasi berbeda, yaitu dari TPA dan hutan mangrove diambil dengan menggunakan metode purposive sampling. Pengambilan sampel pada lokasi TPA dan hutan mangrove dilakukan pada 5 titik yang berbeda dalam satu area yang sama dengan ulangan sebanyak 3 kali, sehingga dalam satu area dapat diperoleh total sampel tanah sebanyak 15 sampel. Sampel tanah diambil pada lapisan atas tanah yang telah digali sedalam 10-15 cm sebanyak 100 Gram dengan menggunakan sekop dan dimasukkan ke dalam ziplock steril yang telah diberi label. Untuk mengetahui berat kering awal plastik, potongan tersebut dikeringkan dengan oven pada suhu 80°C selama 24 jam, sehingga diperoleh berat murni plastik tanpa kandungan air.
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