Two Isoforms of Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1 (UCH-L1) were Down-Regulated in High Metastatic Potential of Human SN12C Renal Cell Carcinoma Cell Clones

Abstract

The SN12C renal cell carcinoma cell clones were parent cell line and 3 clones. SN12C parent cell line was established from a human renal cell carcinoma surgical specimen. The two clones, SN12C-clone 2 and SN12C-PM 6, have higher than the parent cell line. The SN12C-clone 4 has lower than the parent cell line. Using two-dimensional gel electrophoresis, we detected eight proteins showing differential spot intensity between parent cell line and high metastatic clones. And we have identified 5 out of the 8 proteins by using liquid chromatography-tandem mass spectrometry. We found two isoforms of UCH-L1 protein which was shown to be significantly down-regulated in the high metastatic clones. However, the mechanisms of down-regulation of UCH-L1 which were involved in metastasis still remain to be characterized. To clarify the mechanism of UCH-L1 protein expression, further studies will be necessary. Furthermore, we need to examine in what kind of integral mechanism does the down-regulation of UCH-L1 occur.