Abstract
Simple SummarySperm-associated antigen 17 (SPAG17) is a reproduction and skeletal development related gene. This study aimed to identify crucial insertion-deletion (indel) variations, which influence the body measurement traits of goats. Two intronic indels (14 bp and 17 bp indels) were identified by sequencing. In Shaanbei white cashmere goat (SBWC), the different genotypes of the 14 bp indel were markedly associated with goat body height, chest width, body length, and chest depth. The genotypes of the 17 bp indel were significantly associated with body height and chest width. The different combined genotypes were significantly associated with body height and chest width of SBWC and ten traits of Hainan black goat. These results suggested that the 14 and 17 bp indels within SPAG17 can be used in goat growth related traits marker-assisted selection breeding, especially body height. Sperm-associated antigen 17 (SPAG17) gene encodes a multifunctional cytoplasmic protein, which influences not only reproduction but also skeletal development related body measurement traits, especially body height. Thus, this study aimed to identify crucial insertion-deletion (indel) variations, which influence the body measurement traits of goats in large goat populations (n = 1725). As a result, two intronic indels (14 bp and 17 bp indel) were identified by sequencing. For the two indel loci, the distributions of genotypes and alleles were significantly different between the Shaanbei white cashmere goat (SBWC) and the Hainan black goat (HNBG). In SBWC goats, the different genotypes of the 14 bp indel were markedly associated with goat body height, chest width, body length and chest depth. The genotypes of the 17 bp indel were significantly related to body height and chest width. At the two loci, for all seven analyzed traits of SBWC goat, the growth data of DD homozygotes were the worst, which means that the 14 bp insertion and the 17 bp deletion were beneficial and detrimental variations, respectively. Moreover, the combined genotypes were significantly related to body height and chest width of SBWC goats and ten traits of HNBG. These results suggested that the 14 and 17 bp indels within SPAG17 can be used in goat growth related traits marker-assisted selection breeding, especially body height.
Highlights
Growth and development of goats are important factors that influence the developing of the goat industry
Considering the functions of Sperm-associated antigen 17 (SPAG17) on development, this study aimed to reveal the significant genetic variations in the goat SPAG17 gene and investigate their effects on the body measurement traits of two Chinese goat breeds, Shaanbei white cashmere goat (SBWC) and Hainan black goat (HNBG)
D’ value is 0.017, and r2 value is close to 0. These results suggested that the goat SPAG17 14 bp and 17 bp indels are not linked in SBWC goats and HNBG breeds
Summary
Growth and development of goats are important factors that influence the developing of the goat industry. The body measurement traits data of goats directly reflect the body size, structure and development, which are closely related to the physiological function and production performance of goats. The body measurement traits data could be used to guide the scientific raising and breeding of goats. Marker-assisted selection (MAS) is a fast, dependable and feasible breeding method, which is based on the significant variation loci in crucial genes. Identifying crucial genes and markers associated with goat economic traits, such as growth and development, would lay the foundation for goat MAS breeding and assist in the genetic selection of goats. Sperm-associated antigen 17 (SPAG17) plays vital roles in the function and structure of motile cilia [1,2]. A novel testis-specific splice variant of SPAG17 is potentially applicable as immunotherapeutic targets and serologic biomarkers for cancer-testis [6]
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