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Abstract
Bipolaris sorokiniana, a fungal pathogen of cereals, was transformed with the GUS (β-glucuronidase) gene, using a plasmid (pGUS5) containing GUS A with the constitutive promotor GPD-1 and a hygromycin-resistance gene (hph) under the control of the same promotor. Hygromycin resistance was used as the selectable marker. Colonies growing on medium with hygromycin were obtained at a frequency of 6.5×10 -7 per viable fungal protoplast. Nineteen out of 20 tested hygromycin-resistant colonies were also GUS positive. Transformants that showed a stable expression of GUS activity and hygromycin resistance after several conidiation cycles and several months of growth on nonselective medium could be selected [...]
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