Transferring Desirable Genes from Agropyron cristatum 7P Chromosome into Common Wheat.

Mingjie Lu,Cuili Pan,Huanhuan Li,Jinpeng Zhang,Weihua Liu,Xinming Yang,Yong Guo,Yuqing Lu,Lihui Li,Xiuquan Li,Aimin Zhang

doi:10.1371/journal.pone.0159577

Abstract

Wheat-Agropyron cristatum 7P disomic addition line Ⅱ-5-1, derived from the distant hybridization between A. cristatum (2n = 4x = 28, PPPP) and the common wheat cv. Fukuhokomugi (Fukuho), displays numerous desirable agronomic traits, including enhanced thousand-grain weight, smaller flag leaf, and enhanced tolerance to drought. In order to transfer these traits into common wheat, Ⅱ-5-1 was induced by 60Co-γ ray, leading to the creation of 18 translocation lines and three deletion lines. Genomic in situ hybridization (GISH) and fluorescence in situ hybridization (FISH) indicated that multiple wheat chromosomes were involved in the translocation events, including chromosome 2A, 3A, 5A, 7A, 3B, 5B, 7B, 3D and 7D. A. cristatum 7P chromosome was divided into 15 chromosomal bins with fifty-five sequence-tagged site (STS) markers specific to A. cristatum 7P chromosome. Seven and eight chromosomal bins were located on 7PS and 7PL, respectively. The above-mentioned translocation and deletion lines each contained different, yet overlapping 7P chromosomal fragments, covering the entire A. cristatum 7P chromosome. Three translocation lines (7PT-13, 7PT-14 and 7PT-17) and three deletion lines (del-1, del-2 and del-3), which contained the common chromosomal bins 7PS1-3, displayed higher thousand-grain weigh than Fukuho, suggesting that potential genes conferring high thousand-grain weigh might be located on these chromosomal bins. Therefore, wheat-A. cristatum 7P translocation lines with elite traits will be useful as novel germplasms for wheat genetic improvement.

Highlights

Bread wheat (Triticum aestivum L.), serving as a major source of calories, is one of the most important crops in the world
Yield-related genes located on Th. bessarabicum chromosome arm 2JS were transferred into common wheat through wheat-Th. bessarabicum translocation line T2JS-2BSÁ2BL [3]
II-5-1 was derived from the wide hybridization between A. cristatum and Fukuho, followed by self-pollination for six generations

Summary

Introduction

Bread wheat (Triticum aestivum L.), serving as a major source of calories, is one of the most important crops in the world. One effective approach to create novel germplasms is to transfer desirable genes conferring superior agronomic traits into common wheat from its wild relatives. This approach has been successfully employed in wheat improvement. Yield-related genes located on Th. bessarabicum chromosome arm 2JS were transferred into common wheat through wheat-Th. bessarabicum translocation line T2JS-2BSÁ2BL [3]. Two T1BLÁ1RS translocation lines derived from the crosses between common wheat cultivar and rye, showed high stripe rust resistance and good quality [8]. A large number of desirable genes have been successfully transferred into common wheat, resulting in a plenty of novel wheat germplasm. Several translocation lines have been widely used in wheat breading, and the two typical examples were wheat-rye 1BLÁ1RS and wheat-Haynaldia villosa 6VSÁ6AL translocation lines [9,10,11]

Methods

Results

Conclusion