Abstract
Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat (Triticum aestivum L.) worldwide. Agropyron cristatum (L.) Gaertn. (2n = 28, PPPP), one of the wild relatives of wheat, exhibits resistance to stripe rust. In this study, wheat-A. cristatum 6P disomic addition line 4844-12 also exhibited resistance to stripe rust. To identify the stripe rust resistance locus from A. cristatum 6P, ten translocation lines, five deletion lines and the BC2F2 and BC3F2 populations of two wheat-A. cristatum 6P whole-arm translocation lines were tested with a mixture of two races of Pst in two sites during 2015–2016 and 2016–2017, being genotyped with genomic in situ hybridization (GISH) and molecular markers. The result indicated that the locus conferring stripe rust resistance was located on the terminal 20% of 6P short arm’s length. Twenty-nine 6P-specific sequence-tagged-site (STS) markers mapped on the resistance locus have been acquired, which will be helpful for the fine mapping of the stripe rust resistance locus. The stripe rust-resistant translocation lines were found to carry some favorable agronomic traits, which could facilitate their use in wheat improvement. Collectively, the stripe rust resistance locus from A. cristatum 6P could be a novel resistance source and the screened stripe rust-resistant materials will be valuable for wheat disease breeding.
Highlights
Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most devastating and widespread diseases of wheat (Triticum aestivum L.) around the world [1,2,3]
Broadening the genetic base of common wheat by transferring resistance genes from wide relatives may enhance the chance of achieving adequate resistance against stripe rust
Wheat-A. cristatum 6P disomic addition line 4844-12 was immune to the infection with stripe rust (CYR32 and CYR33)
Summary
Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most devastating and widespread diseases of wheat (Triticum aestivum L.) around the world [1,2,3]. Stripe rust, caused by Puccinia striiformis f. Stripe rust has become a major threat to wheat production, causing yield losses of 5–25% [4,5]. There is a need to screen new stripe rust resistance genes for broadening the wheat gene pool and providing new potential resistance genes for the wheat breeding of stripe rust resistance. Wide hybridization is an efficient way of transferring beneficial resistance genes to common wheat. Different resistance genes from distant genera, such as Yr9 from Secale cereal [7,8], Pm21 from Haynaldia villosa [9] and YrH9020 from Psathyrostachys huashanica [10], have been transferred to common wheat, and were effective in enhancing the disease resistance of wheat. The disease-resistant wheat-alien species derivative lines will be used as new wheat resources for breeding new resistant varieties
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