Toxicological studies of additives feed endoforce multi on laboratory animals

Abstract

The creation of new drugs and feed additives is an urgent task that contributes to the growth of the efficiency of the livestock and poultry industries of agriculture, especially during the period of import substitution in the Russian Federation. An experimental study of possible changes in the body of laboratory animals after single or multiple uses of new feed additives is necessary for the toxicological assessment of newly created products. Single intragastric administration of feed additive Endoforce Multi containing live vegetative cells and spores of cultures: Bacillus subtilis B-5449, Bacillus subtilis B-1323, Bacillus subtilis B-3679 - 5.0 109 CFU / ml, at a dose many times higher than recommended, did not lead to the death of laboratory animals, which confirmed the absence of its acute toxicity. Over the entire observation period, the authors did not note any disturbances in the rats’ behavioural reactions and physiological states. Determination of chronic toxicity was carried out for 30 days. The studied suspension of the feed additive was given with water to rats of the experimental groups at doses: 1st - 100 mg, 2nd - 150 mg and 3rd - 200 mg per head. At the end of the empirical study, an increase in the number of erythrocytes by 2.68–3.72 1012/l, haemoglobin - by 41.50–61.58 g/l in laboratory animals of the experimental groups compared with the control ones was revealed, which indicated an increase in blood oxygenation and the organism as a whole. In addition, in the rats of the experimental groups, a decrease in the content of urea in the blood to 3.14 ± 0.53 - 4.68 ± 0.41 mmol / l and creatinine to 32.26 ± 5.18 - 48.13 ± 6.23 µmol/l. The opposite trend was observed when determining the serum activity of alkaline phosphatase, which increased by 39.0–52.6% in intact individuals, which indicated dynamic processes of growth, formation and development of the skeleton. According to the results of the autopsy of the rats of the studied groups, regardless of the dose of the introduction of the feed additive Endoforce Multi, no pathological changes in the colour, consistency and size of the internal organs were registered.