Toxicity Effects of Anthracycline-Converted Herceptin and Azo-Functionalized Fe₃O₄ Nanoparticles on the Heart of Patients with Breast Cancer Based on Echocardiography.

Abstract

The multifunctional nano-carrier system can simultaneously achieve multiple functions such as diagnostic imaging, targeted delivery of anti-tumor drugs, and combined therapy. Application potential Fe₃O₄ magnetic nanoparticles have the characteristics of low toxicity, superparamagnetism and good photothermal properties. Therefore, a multifunctional magnetic nanocarrier with both magnetic targeting and photothermal properties can be prepared by surface modification of Fe₃O₄ o DOX is an anti-tumor drug widely used in clinical treatment, and its severe toxic and side effects greatly limit its application. In this paper, a temperature-sensitive magnetic nanocarrier was first constructed and proved to have good superparamagnetism, photothermal properties, and biocom-patibility Then, Fe₃O₄-Azo-DOX drug-loaded nanoparticles were constructed by covalently bonding DOX. The prepared Fe₃O₄-Azo-DOX nanoparticles have high stability, sensitive photothermal response and low toxicity. Finally, Fe₃O₄-Azo-DOX was applied to the study of combined photother-motherapy and chemotherapy in vitro and in vivo. Based on Fe₃O₄ nanoparticles, a temperature-sensitive Fe₃O₄-Azo nanocarrier was constructed and its related properties were characterized. Furthermore, anthracycline nanodrugs were used in chemotherapy of breast cancer patients, and their effects were analyzed according to echocardiography parameter change. The results show that Fe₃O₄-Azo nanoparticles have a good photothermal heating effect. MCF-7 breast cancer cells were selected as a model to investigate the cytotoxicity of Fe₃O₄-Azo. The results proved that they have excellent biocompatibility and can be used as drug carriers. A Fe₃O₄-Azo nanocarrier was used to load DOX to construct a NIR-responsive nano-drug delivery system. By studying the NIR controlled release of Fe₃O₄-Azo-DOX under different pH conditions, it can be seen that it has NIR-responsive release function and the best release effect at pH 5.7. It was found that LVEF, LVFS, and E/A were significantly lower after chemotherapy than before (P < 0.05), which had a certain clinical value in cardiotoxicity The in vitro antitumor effect of Fe₃O₄-Azo-DOX was studied, and the results showed that the combined effect of photothermal-chemotherapy was significantly better than the photothermal treatment based on Fe₃O₄-Azo carrier alone and the chemotherapy based on free DOX alone.