TOTAL PHENOLIC AND FLAVONOID CONTENT, ANTIOXIDANT ACTIVITY AND ANTIMICROBIAL POTENTIAL OF PHLOMIS PUNGENS WILLD

Yehor Bazavluk,Solomiia Mykytiuk,Nataliya Polish,Roksolana Konechna,Vira Hamada,Volodymyr Novikov

doi:10.23856/3713

Abstract

The objective of the present study was to determine the total content of phenolics and flavonoids in ethanol extracts of Phlomis рungens Willd. The total phenolic content was estimated spectrophotometrically using Folin Ciocalteu method. The total flavonoid content was measured by aluminium chloride colorimetric assay. The antioxidant and antimicrobial effects of the extracts were investigated. The Phlomis рungens Willd can be regarded as a promising natural plant source of antimicrobial and antioxidant effects with high potential for phytopreparations.

Highlights

Despite significant advances in modeling and creation of synthetic drugs, the popularity of herbal therapy is increasing and its competence is expanding
The purpose of our study is to investigate the chemical composition of the ethanol extracts of Phlomis pungens Willd., in particular phenolic compounds and flavonoids and to study their antimicrobial and antioxidant effects
2.3 Determination of total phenolic content The determination was performed using a spectrophotometric analysis using a modified Folin-Ciocalteu method. 1 ml of Folin reagent, 20 ml of distilled water and 3 ml of 20% Na2CO3 solution were added to 1 ml of the analyzed solution, diluted in a ratio of 1:20

Summary

Introduction

Despite significant advances in modeling and creation of synthetic drugs, the popularity of herbal therapy is increasing and its competence is expanding. Medicinal plants contain evolutionarily formed complexes of native substances engaged in complex interactions. Despite the pronounced pharmacological effect of the active substances in herbal preparations, their ultimate therapeutic effect on human organs and functional sytems is due to numerous actions and interactions of all components of the complex of bioactive compounds. Natural drugs are known to have a milder effect than synthetic agents. Undesirable side effects of drugs, including those of synthetic origin, are observed in 10-40% of patients and are one of the obstacles in the development of new drugs.

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