To Estimate the Levels of Serum Vitamins A, E, And C in Cervical Cancer Patients

Abstract

Background
 Cervical cancer continues to be a leading cause of cancer-related mortality among women in developing nations, including India. Worldwide, cervical cancer is the third most frequent malignancy among women. In comparison to urban regions, cervical cancer is more common in rural communities. This investigation find the impact of vitamins (A, E, and C) on cervical cancer severity in SGT Hospital patients was the goal of this study.
 Aim and Objectives
 
 To determine the levels of serum vitamin A,C and E in cervical cancer patients and healthy controls
 To compare the serum level of vitamin A,C and E in patients with cervical cancer and healthy control
 
 Materials and Methods
 This study is conducted in Department of Biochemistry in collaboration with Department of Pathology, and Department of Gynaecology at SGT Hospital, Budhera, Gurugram's Faculty of Medicine and Health Sciences, this is hospital-based cross-sectional case control study. Subjects are 50 healthy volunteers who are age-matched from the general population as the control group. A signed and informed permission is obtained from each individual in both groups once they have been fully informed of the study's objectives. Before beginning the sample collection, institutional ethical committee approval is soughted. Samples are centrifuged at 3000 rpm for 10 minutes; the serum is separated from the clot in the plain tube. The plasma obtained after centrifuging the blood in the lithium heparin tube at 3000 rpm is decanted. Vitamins A and E is examined on zaivik by HPLC method and Vitamin C is examined by ELISA method
 Results
 Patients with cervical cancer had a significantly lower level of a parameter, such as serum vitamin A, C and E when compare with controls. Our study shows that serum vitamin A levels in the patients were 30.2 ±4.0 mcg/dl and 54.7 ±10.6 mcg/dl, respectively, with a p value of <0.05 which are lower when compared with controls. Vitamin E levels were also decreased in study group (4.5±1.5 mcg/dl ) than controls (11.9±2.7 mcg/dl ) having p value <0.05. Vitamin C levels in cases (1.83±0.3) mcg/dl were considerably lower than those in control groups 9.8±2.5 mcg/dl having p value <0.05.
 Conclusion: Our results imply that lower antioxidant vitamin levels may contribute to the aetiology and development of cervical carcinoma, even if the precise mechanisms through which oxidative stress might cause cancer are still unclear.