Abstract
The effect of glucocorticoids on tissue-specific regulation of glucocorticoid receptor mRNA was studied in intact and adrenalectomized rats. Glucocorticoid receptor mRNA was examined by Northern blot hybridization and quantitated by slot blot hybridization using a glucocorticoid cRNA probe. Glucocorticoid receptor mRNA was greatest in the lung with the relative levels in other tissues as follows: spleen, 70%; brain, 55%; liver, 50%; kidney, 43%; heart, 35%; adrenal, 13%; and testis only 8%. A tissue-specific difference in glucocorticoid receptor mRNA accumulation was found after adrenalectomy. There was little change in glucocorticoid receptor mRNA levels in liver and lung, but the brain and kidney demonstrated a 40 and 80% increase in mRNA, respectively. In contrast, dexamethasone treatment resulted in a consistent decrease of 40-60% in the accumulation of glucocorticoid receptor mRNA in all tissues studied. These results provide in vivo evidence for the autoregulation of the glucocorticoid receptor by its homologous ligand and demonstrate the existence of tissue-specific regulation of the glucocorticoid receptor mRNA levels in states of glucocorticoid excess and depletion.
Highlights
Glucocorticoid re- interpretation of these data is complicated by the binding of ceptor mRNA was examined by Northern blot hybridization and quantitated by slot blot hybridization using a glucocorticoid cRNA probe
There was little change in glu- Little information is available on the synthesis of the cocorticoid receptor mRNA levels in liver and lung, glucocorticoidreceptor and theprocess of down regulation of but the brain and kidney demonstrated a 40 and 80% its hormone receptor
In the present study,we have quantitated the ticoid receptor by its homologous ligand and demon- glucocorticoid receptor mRNA levels in a variety of tissues strate the existence of tissue-specific regulation of the and studied the regulation of the glucocorticoid receptor glucocorticoid receptor mRNA levels in states of glu- mRNA in normal animals and afteradrenalectomy and treatcocorticoid excess and depletion
Summary
Northern Blot Hybridization-Poly(A)-enriched RNA was denatured with 1 M glyoxal, 50% dimethyl sulfoxide [20] and electrophoresed through a horizontal 0.8%agarose gel. Densitometric scanning of the autoradiographs demonstrated thatglucocorticoid receptor mRNA in normal animals (Fig. 2, white bars) was most abundant in the lung with relative levels in other tissues of: 70% in spleen, 55% in brain, 50% in liver, 43% in kidney, 35% in heart, 13% in adrenal, andonly 8%in testis (Fig. 2). RNA Slot Blot Hybridization-The total (5and 7 kb) glucocorticoid receptor mRNA from each tissue sample was quantitated by slot blot hybridization. Eter (LKB2202 Ultro Scan) to quantitate theamount of probe that The RNA was obtained 6 h after dexamethasone treatment hybridizated to the glucocorticoid receptor mRNA.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
