Abstract
Plants have remarkable regenerative capacity, which allows them to survive tissue damage after exposure to biotic and abiotic stresses. Some of the key transcription factors and hormone crosstalk mechanisms involved in wound-induced organ regeneration have been extensively studied in the model plant Arabidopsis thaliana. However, little is known about the role of metabolism in wound-induced organ formation. Here, we performed detailed transcriptome analysis and used a targeted metabolomics approach to study de novo organ formation in tomato hypocotyl explants and found tissue-specific metabolic differences and divergent developmental pathways. Our results indicate that successful regeneration in the apical region of the hypocotyl depends on a specific metabolic switch involving the upregulation of photorespiratory pathway components and the differential regulation of photosynthesis-related gene expression and gluconeogenesis pathway activation. These findings provide a useful resource for further investigation of the molecular mechanisms involved in wound-induced organ formation in crop species such as tomato.
Highlights
Plants have remarkable regenerative capacity, enabling them to either repair damaged tissues or regenerate lost organs [1]
We confirmed the absence of preformed shoot apical meristems (SAMs) in these explants, which resulted in rapid sealing of the wound in the apical region (Figure 1c, stage 1)
Since we showed that both photosynthesis and sucrose metabolism are deregulated during wound-induced organ formation, we investigated the relevance of these two factors during de novo organ formation in tomato hypocotyl explants
Summary
Plants have remarkable regenerative capacity, enabling them to either repair damaged tissues or regenerate lost organs [1]. This regenerative capacity allows plants to overcome tissue damage caused by different environmental insults. The many advantages of Arabidopsis thaliana and the wide repertoire of regenerative responses in this species have provided some insight into the molecular mechanisms underlying tissue repair and organ regeneration [2,3]. Key transcription factor families and hormone crosstalk mechanisms involved in plant regeneration have been extensively studied in A. thaliana [5]. Sucrose is frequently chosen in cell and tissue culture media with positive effects on callus proliferation and shoot regeneration, the precise mechanisms underlying these effects are currently unknown [10]
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