Abstract
Background: Investigation for research on the cellular biology and physiology of colon cancer tissues requires viable dissociation of single cells. The amount of tissue and dissociation methods can affect the amount of single cell viability. Inadequate initial tissue has negative effects on data quality by resulting in insufficient quality and the number of cells. 
 Methods: In the context of this study different-weigh and different-textured colon tumor tissues have been evaluated to emphasize the importance of initial tissue properties during the operation of tissue processing and cell isolation success. Necrotic areas were also evaluated with the isolated viable cells and the success of 3D primary culture. 
 Results: Elevated weight of the tissue resulted with more total isolated cells. Necrotic tissues caused low percentage of viable cells. Since resected tissues were bigger than biopsy samples, resected tissues derived primary 3D culture were succesfully maintained the culture.
 Conclusion: To conclude, isolated cells from the bigger and non-necrotic tumor tissues showed better growth pattern for 3D cultures. On the other hand, size was found as a crucial parameter for obtaining more viable cancer cells.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
