Tissue culture and rapid propagation for stems of Cinnamomum camphora

Abstract

To study the effect of naphthalene-acetic acid （NAA） and 6-benzylaminopurine 6-BA on the induction and multiplication of multiple shoots for Cinnamomum camphora during the process of primary culture and subculture, stems with axillary buds which sprouted at the beginning of spring were used as explants. To select the optimum medium for subculture, an orthogonal experiment was designed, and an extreme difference analysis and variance analysis （ANOVA） was used. Results showed that the optimal medium for inducing axillary buds was Murashige and Skoog （MS） + 1.00 mgL－1 6-BA + （0.01－0.10 mgL－1） NAA. The orthogonal experiment and ANOVA showed that the best medium for formation of multiple shoots was MS + 1.00 mgL－1 6-BA + 0.10 mgL－1 NAA, and the optimum medium for proliferation of multiple shoots was MS + 0.50－1.00 mg L－1 6-BA. After the aseptic seedling was transferred to the optimum rooting medium （1/2MS + 1.50 mg L－1 NAA）, the rooting rate reached above 90%. After 15－20 d of hardening, seedlings were transplanted to a mixed matrix with equal vermiculite and peat where the survival rate was greater than 95%. Thus, an efficient breeding technology for C. camphora was studied laying a technical foundation for industrialization of improved varieties. ［Ch, 2 fig. 5 tab. 13 ref.］