Micropropagation and assessment of somaclonal variation in Galanthus transcaucasicus in vitro plantlets

Narges Asadi,Seyyed Javad Mousavizadeh,Hossein Zarei,Seyyed Hamidreza Hashemi-Petroudi

doi:10.1590/2447-536x.v27i4.2320

Abstract

Abstract In vitro culture of twin-scaling explants of Galanthus transcaucasicus with different concentrations of plant growth regulators (PGRs) including 0.5, 1, 2, 3, 4, 6, 8, and 10 mg L-1 naphthaleneacetic acid (NAA) and 0.5, 1, 2, 3, and 4 mg L-1 benzyladenine (BA) was studied. After 18 weeks, the number of regenerated bulblets and intensity of callus was measured. Subsequently, bulblets were transferred to a medium with 0.5, 1, 2, 3, and 4 mg L-1 NAA and 0.5, 1, 2, 3, and 4 mg L-1 BA and, after 15 weeks, the bulblets length and diameter were measured. The highest intensity of callus was obtained on 4 mg L-1 NAA or 8 mg L-1 NAA with 1 mg L-1 BA. The highest number of regenerated bulblets was detected with 6 mg L-1 NAA and 2 mg L-1 BA. The highest diameter of bulblets occurred on four mgL-1 NAA (9.4 mm), while the lowest was observed on 0.5 mg L-1 BA (1.83 mm). The analysis of genetic variation using ISSR revealed that there was no somaclonal variation among the regenerated plants from BA and low level of NAA, but there was a significant somaclonal variation at high concentrations of NAA.

Highlights

Galanthus spp. (Snowdrops) are perennial bulbous plants belonging to the Amaryllidaceae tribe Galantheae whose seed germination is difficult in natural conditions (Larsen et al, 2010; Newton et al, 2013)
This study aimed to identify the effects of plant growth regulators (PGRs) (NAA and BA) concentrations on G. transcaucasicus bulblet and callus regeneration
Means followed by the same letter within columns are not significantly different at p ≤ 0.05 level according to Duncan multiple range test (DMRT)

Summary

Introduction

Galanthus spp. (Snowdrops) are perennial bulbous plants belonging to the Amaryllidaceae tribe Galantheae whose seed germination is difficult in natural conditions (Larsen et al, 2010; Newton et al, 2013). (Snowdrops) are perennial bulbous plants belonging to the Amaryllidaceae tribe Galantheae whose seed germination is difficult in natural conditions (Larsen et al, 2010; Newton et al, 2013). The Amaryllidaceae family is potentially important from an economic viewpoint both for the chemical pharmaceutical industry and the ornamental plants market. It is identified because of its two leaves as well as pendant white flowers with six perianth segments; three outer and inner perianth segments (Rønsted et al, 2013). The bulb is formed very slowly over too long periods of time, especially in comparison with Liliaceae, Iridaceae and Amaryllidaceae families in the natural environment (Çiğ, 2015).

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