Effects of carbendazim on &lt;em&gt;In vitro&lt;/em&gt; micropropagation and flowering of pepper elder herbal plant

Muneeb M. Musthafa,Vidya Devanathadesikan Seshadri,Abdul Bakrudeen Ali Ahmed,Vijay Lobo Vidhyavathi,Faiz M. M.T. Marikar,Teoh Lydia

doi:10.4038/tare.v22i1-2.5484

Abstract

The present study reports on application of carbendazim for micropropagation and in vitro flowering of Peperomia pellucida. In vitro multiplication of shoot tips and axillary nodal explants were used in 25-100 mgL-1 carbendazim in 70% alcohol treatment for 5 min in MS (Murashige and Skoog) medium and MS hormone medium The explants were cultured on MS medium supplemented with auxins, cytokinins and additives (Casin hydrolyte and glutamine) used to proliferate the shoots. The maximum number of shoots (21.5/shoot tip) were developed on MS medium supplemented with 2.0 mgL-1 KIN (Kinetin) 0.5 mgL-1 NAA (α- Naphthalene acetic acid), 100 mgL-1 casein hydrolysate and 100 mgL-1 glutamine additives. The highest number of roots per shoot (17.6) and highest rooting frequency (94.2%) were obtained when the adventitious shoots were inoculated on MS medium with 1.5 mgL-1 IAA (Indole-3-acetic acid). The highest number of shoot development (75.4 %) was achieved on MS medium supplemented with 2.0 mgL-1 KIN 2.0 + 0.5 mgL-1 ABA (Abscisic acid) with 5% sucrose. It was found that MS medium devoid of 1.5 mgL-1 IAA promote the root formation and maturation of in vitro flower in P. pellucida. Acclimatized plantlets were transferred to botanical garden and the characters compared with mother plant of P. pellucida.

Highlights

Peperomia pellucida is a medicinal plant belongs to the family Piperaceae and commonly known as pepper elder, shining bush plant and man to man
The aim of this study was to investigate the potential of in vitro culture of P. pellucida, i) Surface sterilization with suitable surfactant for mass propagation of clonal material, in vitro milieu to engage in other applied biotechnologies; ii) plant growth regulators (PGRs) induced the multiple shoot initiation and proliferation with suitable organic elicitors followed by root induction and acclimatization from shoot tip and axillary node explants; iii) this study deals with the development of in vitro flower system from shoot tip explants and the subsequent transplantation of the plantlets
Explant Surface sterilization Explants were surface sterilized with different surfactants and carbendazim (0.1, 0.2, 0.4, 0.6 and 0.8% prepared by 70% ethanol)

Summary

Introduction

Peperomia pellucida is a medicinal plant belongs to the family Piperaceae and commonly known as pepper elder, shining bush plant and man to man. It is a tropical annual fibrous shallow-rooted herb, which grows as succulent and erect plant (Wagner et al 1999; Majumder 2012). The stem part is fleshy, round, delicate and glabrous (Majumder 2012), whereas bud like structures attached to several fruiting spikes. The plant is used in traditional medicine to cure conditions like abdominal pain, boils, colic fatigue, abscesses, acne, convulsions, fever, gout, headache, renal disorders, , conjunctivitis, skin diseases, breast cancer rheumatic joint pain and to lower blood cholesterol level (Khan et al 2010). Peperomin showed anti-cancer activity, arylpropanoids shown antifungal activity (Wei et al 2011) and flavonoids components showed the antioxidant, anti-neoplastic, antiulcer, anti-inflammatory and anti-microbial activities (Majumder 2011)

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