Abstract

The effects of timing in phosphate application on plant growth and arsenic removal by arsenic hyperaccumulator Pteris vittata L. of different ages were evaluated. The hydroponic experiment consisted of three plant ages (A 45d, A 90d and A 180d) and three P feeding regimens (P 200+0, P 134+66 and P 66+134) growing for 45 d in 0.2-strength Hoagland–Arnon solution containing 145 μg L −1 As. While all plants received 200 μM P, P was added in two phases: during acclimation and after arsenic exposure. High initial P-supply (P 200+0) favored frond biomass production and plant P uptake, while split-P application (P 134+66 and P 66+134) favored plant root production. Single P addition favored arsenic accumulation in the roots while split-P addition increased frond arsenic accumulation. Young ferns (A 45d) in treatment P 134+66 were the most efficient in arsenic removal, reducing arsenic concentration to below 10 μg L −1 in 35 d. The results indicated that the use of young ferns, coupled with feeding of low initial P or split-P application, increased the efficiency of arsenic removal by P. vittata.

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