Time‐Resolved Shotgun Lipidomic Analysis of Murine Astrocyte Cells Exposed to Airborne Particulate Matter

Abstract

Millions of people around the world that are affected by neurodegenerative disease. A potential correlation has been found between neurodegenerative disease and particulate pollution. Several studies have shown oxidative stress in the brain and induction of neuroinflammation due to airborne particulate matter. Our lab has shown that urban dust induces necrosis, and diesel exhaust particulate matter induces apoptosis. I studied the the lipid profile of murine astrocyte cells exposed to urban dust and diesel exhaust at several different time points. I used shotgun lipidomics to identify all the lipid chemical species present in the cell samples. The samples were run through the 12T Fourier Transform Ion Cyclotron Resonance Mass Spectrometer (COSMIC). A key difference between the treated and untreated samples was the presence of certain ceramides. We believe these ceramides might be associated with ceramide signaling pathways in cell death.Support or Funding InformationResearch reported in this publication was supported by an Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health under grant number P20GM103443. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of HealthThis abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.