Abstract
This article provides an introduction to Fourier transform-based mass spectrometry. The key performance characteristics of Fourier transform-based mass spectrometry, mass accuracy and resolution, are presented in the view of how they impact the interpretation of measurements in proteomic applications. The theory and principles of operation of two types of mass analyzer, Fourier transform ion cyclotron resonance and Orbitrap, are described. Major benefits as well as limitations of Fourier transform-based mass spectrometry technology are discussed in the context of practical sample analysis, and illustrated with examples included as figures in this text and in the accompanying slide set. Comparisons highlighting the performance differences between the two mass analyzers are made where deemed useful in assisting the user with choosing the most appropriate technology for an application. Recent developments of these high-performing mass spectrometers are mentioned to provide a future outlook.
Highlights
Mass spectrometers have been used for a long time in a variety of biological applications
Multidimensional liquid chromatography (LC) separations have an important role to play in proteomics applications for reducing sample complexity, and complement well the high dynamic range of detection in an acquisition offered by Fourier transform-based mass spectrometers (FTMS) instruments
The current article does not intend to be an exhaustive review paper on FTMS techniques; it is intended as teaching material for scientists without a physics background to assist them with understanding the mass spectrometry tools they are called to use in their everyday laboratory work
Summary
Michaela Scigelova‡¶, Martin Hornshaw§, Anastassios Giannakopulos‡, and Alexander Makarov‡. The FTICR and Orbitrap analyzers outperform any other commonly used mass spectrometer with respect to the maximum mass resolution and accuracy routinely achievable even for small numbers of ions. Both these mass spectrometers share certain features, such as an image current detection system and the application of Fourier transform mathematical operations for generating mass spectra from time domain tran-. Additional information other than just the accurate mass measurement will be needed to obtain correct elemental composition This includes, among others, Molecular & Cellular Proteomics 10.7.
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