Abstract
The Intermediate Voltage Electron Microscopy and Biomedical Image Analysis facility at the University of Pennsylvania is a National Resource supported by NIH and provides users with facilities for transmission electron microscopy and digital image processing. The facility comprises a JEOL 4000EX transmission electron microscope with a 360 degree goniometer specimen holder, a VAX 11/750 computer, and a Raster Technologies Inc. Model One/380 Graphics work station with two high resolution 1280x1024 pixel color video display monitors for stereoscopic display. We are using this facility to examine the morphology of fibroblasts migrating through fibrillar collagen gels: these matrices closely model the environments encountered by migratory cells during embryonic morphogenesis.The identification and three dimensional localization of structures in stereoscopic electron micrographs of thick sections of cells can be hampered by diffuse boundaries and by the superposition of details with similar electron density.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call