Three acidic polysaccharides derived from sour jujube seeds protect intestinal epithelial barrier function in LPS induced Caco-2 cell inflammation model

Abstract

Normal intestinal epithelial barrier function plays a key role in the prevention of many diseases such as infectious enteritis, inflammatory bowel disease, obesity, etc. In this study, three novel acidic polysaccharides ZY-2, ZY-3 and ZY-4 were isolated from sour jujube (Ziziphus jujuba Mill. var. Spinosa) seeds and purified by DEAE Sephrose Fast Flow gel. The molecular weight of ZY-2, ZY-3 and ZY-4 was 7.76 kDa, 10.71 kDa and 8.31 kDa respectively, mainly composed of different proportions of mannose, rhamnose, glucose, glucuronic acid, galacturonic acid, galactose, xylose and arabinose. 1H NMR and Congo red experiment results showed that the three polysaccharides mainly contained both α-type and β-type glycosidic bonds with obvious triple helix structural traits. The polysaccharides could up-regulate the expression levels of occludin and ZO-1 in LPS-induced inflammation Caco-2 cells, and reduce IL-6, IL-8, IL-1β and TNF-α significantly. In conclusion, the acidic polysaccharides from sour jujube seeds exhibited great potential in protection intestinal epithelial barrier function through anti-inflammatory effects.