In vivo evaluation by oral administration of chitosan combined with bioactive glass against cadmium-induced toxicity in rats

Abstract

Bioactive glass and chitosan are biomaterials widely used for orthopedic applications, notably as bone grafts. Although these biomaterials show promising therapeutic properties, no research has yet examined their potential for oral administration in soft tissue protection, particularly against metal toxicity. The aim of our study was to evaluate the potential of chitosan from cuttlefish (CHS) bone combined with bioactive glass (BG) against Cadmium-induced toxicity in rats. Cadmium (Cd), a heavy metal that accumulates in tissues, causes various disorders. Experiments were carried out on rats intoxicated acutely by oral administration of Cd (20 mg/kg body weight) and/or concomitantly with oral administration of CHS/BG (100 mg/kg body weight) for 7 days. Using pathophysiological and biochemical tests, we evaluated the detoxifying effect of orally administered CHS/BG against Cd toxicity. Our results showed, for the first time, a significant detoxifying effect of CHS/BG against Cd-induced toxicity in rats. Treatment with CHS/BG protected rats against the harmful effects of Cd by reducing lipid peroxidation levels and enhancing antioxidant enzyme activities. In addition, it helped restore phosphocalcic balance and protect liver, kidney and brain function. Remarkably, it also reduced Cd levels in the liver, kidneys and brain, as well as in the bones of rats. These results show that oral administration of CHS/BG has a strong therapeutic potential on tissues through detoxification of cadmium-exposed rats.