Thein vitroisolation ofBlastomyces dermatitidisfrom a woodpile in north central Wisconsin, USA

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Environmental isolations of the dimorphic fungus, Blastomyces dermatitidis, the aetiologic agent of blastomycosis are rare, and have usually involved animal inoculation techniques. We report the in vitro isolation of B. dermatitidis from a woodpile in November 1997, from a private property in a highly endemic area of north central Wisconsin, USA. The woodpile was 73 m from the Wisconsin River and 5 m from a kennel which had housed nine dogs over the past 14 years, four of which had been diagnosed with blastomycosis. One of 19 samples from the property yielded B. dermatitidis after 37 degrees C incubation in a neutral aqueous solution of allantoin, Tween-80, potassium phosphate, magnesium sulphate, penicillin and streptomycin followed by plating on yeast-extract phosphate agar at 20 degrees C. Refinements of this technique may help further elucidate the ecological niche of B. dermatitidis.