The Toxic Effect of Bisphenol A on Male Rat Testicles and The Possible Protective Value of Propolis and Vitamin E

Abstract

This study aimed to examine the impact of propolis (Pro) on various physiological parameters in male rats experiencing induced testicular dysfunction by Bisphenol A. Five groups of ten male rats apiece were created by random selection from the rats. Group 1 the rats, which performed as the group under negative control, received normal food and water. In Group 2, rats were subjected to intraperitoneal administration of 0.2 mL of corn oil, utilized as the vehicle for bisphenol A (BPA), thereby serving as the vehicle control group. For Group 3, rats were administered in corn oil, BPA dissolved at a dosage of 50 mg/kg body weight administered three times a week for three weeks using an intraperitoneal injection. In Group 4rats received a dose of 250 mg/kg body weight of propolis (protected) orally via a gavage needle. Subsequently, intraperitoneal injections are administered to them from BPA delivered three times weekly for three weeks, the dosage is 50 mg per kilogram of body weight dissolved in corn oil. In Group 5, rats received protection with vitamin E orally, at a dose of 100 mg/kg body weight, using a gavage needle. The intraperitoneal injection of BPA, dissolved in corn oil at a dose of 50 mg/kg body weight, was then given three times a week for three weeks. After three weeks, animals were sacrificed, and testes and blood were gathered for additional analysis. Superoxide Dismutase (SOD), Sperm Viability, General motility, Progressive motility, and Morphology, were all significantly reduced in the BPA group compared to the control groups, according to the results. While malondialdehyde (MDA) increased in the BPA group compared to the control groups. In conclusion, propolis enhanced physiological parameters adversely affected by BPA, suggesting its potential as a protective agent against BPA-induced testicular dysfunction.