Abstract
AbstractThe enzymes responsible for the metabolism of methylated xanthines suah as theophylline, theobromine, and caffeine have until now escaped identification (1, 2). However, a sensitive radiometric method of analysis of theophylline metabolism (3) was made possible by the synthesis and purification of 8‐14C‐theophylline (14C‐Theo). 14C‐Theo was synthesized by the incorporation of 14C from 14C‐formic acid into the 8‐position of the purine ring. Purification of the 14C‐Theo was accomplished by subjecting the crude produot to cation‐exchange column chromatography with AG50W‐X12, 50–100 mesh resin of the hydrogen ion form.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.