Abstract
Incorporation of 14C from various 14C-labelled compounds into vitamin B6 (abbreviate as B6) in a cell-suspension of B6-producing bacteria, Achromobacter cycloclastes A.M.S. 6021, was studied by using a newly designed purification procedure of the radioactive B6. The procedure consisted of Sephadex G–25 gel filtration, Dowex 50W–X8 column chromatography, Amberlite CG–50 column adsorption, powdered cellulose partition column chromatography, crystallization and sublimatography. It was observed that the labels both from 1,3- and from 2-labelled glycerol were clearly incorporated into B6 and the label of 14C-labelled γ-aminobutyric acid was also incorporated. The incorporation of 14C from 14C-labelled glycerol or γ-aminobutyric acid was affected by the addition of cold γ-aminobutyric acid or glycerol. The implication of these compounds as precursors of B6 was discussed.
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