The state of microcirculation, the hemostasis system, and level of proteins irisin and CCL11 in young and old rats

Abstract

A significant role in the development of cardiovascular pathology in old age belongs to age-related changes in the microvasculature and the hemostatic system. The development of age-related diseases is influenced by both the level and the ratio of the proteins «youth» (irisin) and «old age» (CCL11). To carry out effective prevention of the occurrence of these diseases, it is necessary to study the characteristics of the functioning of an aging organism. The aim is to assess the state of the microvasculature, the hemostasis system, the level of the proteins irisin and the chemokine CCL11 in young and old rats. Young and old male rats (28 individuals) of the Wistar line were used in the experiment. The state of the microvasculature, the hemostasis system of animals, the level of the proteins irisin and CCL11 were studied. In old rats, compared with young animals, at the microcirculatory level, spasm of arterioles was observed on the background of a decrease in nutritional blood flow. An increase in the aggregation activity of platelets in aged animals was accompanied by hypercoagulation, recorded using clotting and global techniques, as well as a decrease in the anticoagulant activity of blood plasma and activation of fibrinolysis. In addition, in older animals, a decrease in the level of the irisin protein was recorded, while the chemokine CCL11 increased. So, with increasing age, there is a significant deterioration in the state of the microvasculature, hypercoagulative shifts in the hemostasis system and unfavorable changes in the level and ratio of proteins of "outh" and "old age".