Abstract
An ATP analog, TuTP, was tested as a substrate for Escherichia coli RNA polymerase (nucleoside triphosphate: RNA nucleotidyltransferase, EC 2.7.7.6) activity using either deoxypolynucleotides with ordered nucleotide sequences or natural DNA's as a template. TuTP could replace ATP, and poly (Tu-G) was synthesized instead of poly (A-G) when poly d(T-C)·d(A-G) was used as a template. The rate of synthesis of poly (Tu-G) was slower than that of poly (A-G), although the final yield in both reactions was the same. However, when other deoxypolynucleotides such as poly d(T-G) · d(A-C), poly d(A-T), poly T or natural DNA's were used as templates, little or no TuTP was utilized. It is concluded, therefore, that efficiency of incorporation of TuTp is determined not only by its own properties, but also by the structure of the deoxypolynucleotide used as a template. The possible mechanism of the selective incorporation of TuTP is discussed.
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