The Role of Vitamin B12 on Adenosine Monophosphate-activated Protein Kinase in Anemic Patients

Abstract

Background: Anemia affects people at all stage of their lives, but it is most common in pregnant women and young children. Iron deficiencies are metabolic stress since it affects both the abilities to provide oxygen to tissue and the abilities to use oxygen (mitochondrial capability impairment). Vit.B12 plays role in the metabolism of AMP-activated protein kinase which considers as protection against inflammation in-vitro and in-vivo. Methodology: This study includes 30 samples of the patient and 30 samples as control groups, by centrifugation, blood samples were separated and stored at minus twenty centigrade. Different questions were asked of all patients and control groups. like duration of anemia, malabsorption, malnutrition, history, drugs, weight, height, and smoking Results: The results showed that the mean value of height levels in patients (162.2 ± 4.495 ) no significant difference (p-value = 0.6) as compared to control groups (165.6 ± 5.359) also in weight levels showed no significant difference (p-value = 0.9) between control (69.3 ± 3.418) and patients groups (63.8 ± 4.654). In vit.B12 levels, the mean value of patients (295.923 ± 162.241) showed a significant difference (p-value = 0.012*) as compared with mean of control groups (482.2 ± 158.12). In transferrin levels, there was a significant difference (p-value=0.04**) in vit.B12 levels between mean of patients (29.307 ± 11.604) and mean of control groups (42.4 ± 8.711). In addition to that, the mean value of Hb levels in patients (9.7 ± 2.200) showed significant difference (p-value = 0.03*) as compared to control groups (14.1 ± 0.94), finally, PCV levels showed a significant difference (P-value = 0.02*) in Polycythemia Vera (PCV) levels between mean of patients (43.3 ± 3.82) and mean of control groups (30.1 ± 6.601). Conclusion: In this study, further demonstrate that vit.B12 plays role to decrease inflammations by macrophage inhibition; we identify the mechanism of AMP pathway mediate vit.B12 action of anti-inflammations, and reported the relation between vit. B12 and AMP-activated protein kinase (AMPK) phosphorylation, therefore vit.B12 is considered as a novel action of anti-inflammatory reagent responses.