The role of Na/I symporter, thyroid peroxidase and type 1 iodothyronine deiodinase gene in the pathogenesis of hashimoto's thyroiditis coexistent with papillary thyroid carcinoma

Abstract

Objective To investigate the role of Na/I symporter (NIS),thyroid peroxidase (TPO) and type 1 iodothyronine deiodinase (DIOI) mutation in the pathogenesis of Hashimoto' s thyroiditis (HT) coexistent with papillary thyroid carcinoma (PTC).Methods Thirty-four HT patients with PTC were selected as subjects and 26 cases of HT as controls.The genomic DNA of the specimens was extracted.By using polymerase chain reaction (PCR),interesting fragments were obtained and sequenced.Results Among 34 patients of HT with PTC,no PCR products were found with the mutation in the NIS gene.Ten out of 34 patients and 2 out of 26 controls had 6 TPO point mutations and 1 insertion mutation,and 5 of out of 34 patients and 1 out of 26 controls had 3 DIOI point mutations.Statistical analysis showed there was significance in mutation positivity between HT with PTC and HT.In addition,mutation positivity had significant difference between patients with different thyroid function in HT and PTC group ( P ＜ 0.05 ).Conclusion It is possible that TPO and DIOI gene mutation had a potential molecular biological link with the two diseases. Key words: Na/I symporter; Thyroid peroxidase; Hashimoto's thyroiditis; Papillary thyroid carcinoma