Study of BRAFV600E in the correlation between papillary thyroid carcinoma and Hashimoto's thyroiditis

Abstract

Objective To explore the expression of BRAFV600E in papillary thyroid carcinoma and Hashimoto's thyroiditis,and investigate the correlation between papillary thyroid carcinoma and Hashimoto's thyroiditis.Methods The paraffin embedded tissue specimens of pathologically confirmed thyroid disease during 2012.1-2014.6 in the Air Force General Hospital were collected,including 40 cases of papillary thyroid carcinoma,20 cases of Hashimoto' s thyroiditis,20 cases of papillary thyroid carcinoma with Hashimoto's thyroiditis and 20 cases of thyroid adenoma.Immunohistochemistry (Envision method) was used to detect the expression of BRAFV600E and semi quantitative analysis was used to compare the difference of different thyroid diseases.Results The positive rate of BRAFV600E was 77.5％ (31/40) in papillary thyroid carcinoma and 45％ (9/20) in Hashimoto' s thyroiditis (P =0.012).In papillary thyroid carcinoma with Hashimoto's thyroiditis,the positive rate of BRAFV600E in thyroid papillary carcinoma tissue was 70.0％ (14/20),which was not different from thyroid papillary carcinoma (P =0.156) ; and the positive rate of BRAFV600E in Hashimoto's thyroiditis tissue was 55.0％ (11/20),which was not different from Hashimoto's thyroiditis(P =0.527).The positive rate of BRAFVE600 in metastatic papillary thyroid carcinoma was 88.2％ (15/17),which was higher than that in papillary thyroid carcinoma without metastasis (69.5％,16/23,P =0.030).There was no expression of BRAFV600E in thyroid adenoma.Conclusion Papillary thyroid carcinoma and Hashimoto's thyroiditis have a higher BRAFV600E mutation rate. Key words: Thyroid papillary carcinoma; Hashimoto's thyroiditis; BRAFV600E